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Thrombin and IgE antigen induce formation of inositol phosphates by mouse E‐mast cells
Author(s) -
Baranes D.,
Liu F.T.,
Razin E.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81341-2
Subject(s) - thrombin , chemistry , immunoglobulin e , antigen , stimulation , extracellular , inositol , inositol phosphate , in vitro , microbiology and biotechnology , biochemistry , biology , immunology , endocrinology , antibody , receptor , platelet
Stimulation of murine chondroitin sulfate E containing mast cells (E‐MC) in vitro either by thrombin or immunologically resulted in a rapid formation of inositol phosphates (IPs). Increase in all of the three IPs (IP 1 IP 2 and IP 3 ) could be detected 20 s after stimulation. The depletion of Ca 2+ from the medium resulted in more than 80% reduction in β‐hexosaminidase release from either thrombin or IgE antigen stimulated cells. However, both thrombin and IgE antigen increased the formation of IP 3 under these conditions independent of the presence of extracellular Ca 2+ .