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A telomere binding protein in the macronuclei of the hypotrichous ciliate Stylonychia lemnae
Author(s) -
Steinhilber Wolfram,
Lipps Hans Joachim
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81333-3
Subject(s) - telomere , macronucleus , nitrocellulose , ciliate , microbiology and biotechnology , chemistry , dna , gene , biology , biochemistry , genetics , membrane
Macronuclear proteins from the hypotrichous ciliate Stylonychia lemnae were isolated, separated on SDS‐PAGE and electroblotted onto nitrocellulose filters. Filter binding assays with 32 P‐labeled macronuclear DNA and the synthetic telomere sequence (C 4 A 4 /G 4 T 4 ) in combination with competition experiments revealed strong preference of a protein with a molecular mass of approx. 40 kDa to the terminal sequence of macronuclear gene‐sized molecules.