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Use of fluoride ion as a probe for the guanine nucleotide‐binding protein involved in the phosphoinositide‐dependent neutrophil transduction pathway
Author(s) -
Strnad Colette F.,
Parente Janice E.,
Wong Kenneth
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81332-1
Subject(s) - chemistry , cytosol , signal transduction , gtp' , g protein , pertussis toxin , inositol , biochemistry , fluoride , receptor , biophysics , microbiology and biotechnology , biology , enzyme , inorganic chemistry
Fluoride activation of neutrophils was found to be associated with phosphoinositide turnover, as monitored by the time‐dependent accumulation of inositol phosphates. Unlike phosphoinositide turnover induced by the chemotactic peptide, formylmethionylleucylphenylalanine, that induced by fluoride was not inhibited by pretreatment with pertussis toxin. The translocation of protein kinase C activity from the cytosolic to the membrane compartment was also observed in fluoride‐stimulated cells. We have proposed that the mode of action of this halide ion involves interaction with a GTP‐binding protein which serves as an intermediary unit between the receptors for inflammatory sttimuli and the phosphoinositide‐specific phosphodiesterase.