Premium
Segment α 182‐198 of Torpedo californica acetylcholine receptor contains a second toxin‐binding region and binds anti‐receptor antibodies
Author(s) -
Mulac-Jericevic Biserka,
Atassi M.Zouhair
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81225-x
Subject(s) - torpedo , acetylcholine receptor , receptor , peptide , chemistry , toxin , muscarinic acetylcholine receptor m5 , acetylcholine , biochemistry , nicotinic acetylcholine receptor , antibody , binding site , microbiology and biotechnology , muscarinic acetylcholine receptor m3 , biology , pharmacology , immunology
The area around Cys‐192 and Cys‐193 is thought to be a functionally important part of the α‐subunit of the acetylcholine receptor. We have synthesized peptide α 182‐198 of the α‐chain of the Torpedo californica acetylcholine receptor and investigated the binding to the peptide of α‐bungarotoxin, cobratoxin and antibodies raised against acetylcholine receptor. The results showed that the synthetic peptide α 182‐198 contains a second toxin‐binding region and also binds a considerable fraction of anti‐receptor antibodies. We also report here the toxin‐binding activity of synthetic peptide a 125–148 of the human acetylcholine receptor which has been previously localized as a toxin‐binding region in the α‐chain of the Torpedo receptor.