Premium
Existence of antizyme and ornithine decarboxylase‐antizyme complex in RK13 kidney cells
Author(s) -
Murakami Yasuko,
Marumo Miya,
Hayashi Shin-ichi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81221-2
Subject(s) - ornithine decarboxylase antizyme , ornithine decarboxylase , kidney , chemistry , biochemistry , biology , enzyme , endocrinology
It has been reported that ‘antizyme’, a protein inhibitor of ornithine decarboxylase (ODC) induced by its product, is not found in rat or mouse kidney. We determined whether antizyme was present in rabbit kidney cells (RK13) in culture. Antizyme could be induced in these cells by putrescine treatment, a substantial portion being in the particulate fraction in contrast with hepatic antizyme. Furthermore, ODC‐antizyme complex was present even in untreated cells. Pretreatment of cells with putrescine increased the relative amount of ODC‐antizyme complex and accelerated decay of ODC. These results support the ubiquitous existence of antizyme and its role in ODC degradation.