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Null and electrophoretic mobility mutants in the structural gene for L‐lactate dehydrogenase of Saccharomyces cerevisiae
Author(s) -
Rush Karen,
Sbragia Ralph,
Wills Christopher
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81190-5
Subject(s) - mutant , saccharomyces cerevisiae , lactate dehydrogenase , biochemistry , enzyme , dehydrogenase , microbiology and biotechnology , chemistry , biology , electrophoresis , gene
A mutant lacking L‐lactate dehydrogenase (EC 1.1.2.3) of Saccharomyces cerevisiae was isolated by its inability to grow on minimal medium with L‐lactate as a carbon source. A simple activity gel assay for visualization of this enzyme and the two D‐lactate dehydrogenases in this organism (EC 1.1.2.4 and 1.1.1.28) was developed. This enabled us to screen spontaneous and ethylmethanesulfonate‐induced back mutants for electrophoretic mobility. Two mutants with a mobility faster than that of the wild type were isolated, and proved to be allelic to the L‐lactate dehydrogenase negative mutant.