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Differential expression of fucosyl GM1 and a disialoganglioside with a NeuAcα2‐6Ga1NAc linkage (GD1e) in various rat ascites hepatoma cells
Author(s) -
Iwamori M.,
Sunada S.,
Ishihara E.,
Moki M.,
Fujimoto S.,
Nagai Y.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81186-3
Subject(s) - ascites , immunostaining , ganglioside , sarcoma , biology , cell culture , enzyme , cell , tumor cells , biochemistry , chemistry , microbiology and biotechnology , immunohistochemistry , cancer research , pathology , immunology , genetics , medicine
A distinct difference in ganglioside composition among various rat ascites hepatomas and Yoshida sarcoma was observed on TLC‐immunostaining with anti‐fucosyl GM1 antibody, and chemical and enzymatic analyses. Yoshida sarcoma and ascites hepatomas, AH13, AH66F and AH66, but not the other 9 tumor cell lines investigated, specifically contained a disialoganglioside, NeuAcα2‐3Ga1β1‐3(NeuAcα2‐6)Ga1NAcβ1‐4Galβ1‐4G1cβ1‐1ceramide (GD1e), whereas the 9 ascites hepatoma cells without GD1e contained fucosyl GM1. The differential expression of fucosyl GM1 and GD1e in various tumor cell lines indicates that different cell lineages express distinct metabolic pathways for gangliosides, and that the gangliosides are useful markers for distinguishing tumor cell lines.