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Somatostatin and α 2 ‐adrenergic agonists selectively inhibit vasopressin‐induced cyclic AMP accumulation in MDCK cells
Author(s) -
Friedlander Gerard,
Amiel Claude
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81180-2
Subject(s) - somatostatin , vasopressin , medicine , endocrinology , chemistry , pertussis toxin , adenylate kinase , cyclase , glucagon , adrenergic , hormone , g protein , biology , receptor , stimulation , biochemistry
The effect of somatostatin and α 2 ‐adrenergic agonists on cyclic AMP accumulation was examined in MDCK cells, grown in denned medium. These hormones inhibited vasopressin‐induced cyclic AMP formation, without affecting either the basal or the glucagon‐ and prostaglandin E 2 ‐stimulated level. Pretreating the cells with pertussis toxin, or incubating them with MnCl 2 at a low concentration reversed the effect of somatostatin and α 2 ‐agonists. These results suggest that somatostatin and norepinephrine could selectively modulate the renal effect of vasopressin, via the inhibitory regulatory subunit (N i ) of adenylate cyclase.