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Decrease in myosin light chain kinase activity of rabbit fast muscle by chronic stimulation
Author(s) -
Klug G.A.,
Houston M.E.,
Stullo J.T.,
Pette D.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81167-x
Subject(s) - myosin light chain kinase , myosin , stimulation , immunoglobulin light chain , chemistry , skeletal muscle , biology , medicine , biochemistry , endocrinology , antibody , immunology
Analysis of myosin light chain kinase (MLCK) activity in tibialis anterior muscles of the rabbit revealed that chronic stimulation at a frequency of 10 Hz for 24 h per day reduced the enzyme activity in a timedependent manner. Since fast twitch muscle contains significantly more myosin light chain kinase than slow twitch muscle, the observed reductions are consistent with the type of fast‐to‐slow transformation observed for other type‐specific muscle characteristics. The present data also indicate that the stimulation‐induced decrease in MLCK activity precedes the fast‐to‐slow conversion of the myosin molecule as judged by pyrophosphate‐polyacrylamide gel electrophoresis.