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Activation of chicken liver fructose‐1,6‐bisphosphatase by oxidized glutathione
Author(s) -
Han Peter,
Hang David,
Emmett Nerimiah,
Han Grace,
Johnson Joe
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81166-8
Subject(s) - fructose 1,6 bisphosphatase , glutathione , chemistry , fructose , biochemistry , enzyme
Treatment of chicken liver fructose‐1,6‐bisphosphatase with oxidized glutathione (GSSG) leads to an increase in activity. This activation is markedly enhanced if treatment is performed in the presence of AMP or Mn 2+ . The effects of AMP and Mn 2+ appear to be synergistic. The maximal activation is over 13‐fold and is accompanied by the disappearance of 4 sulfhydryl groups per molecule of enzyme. Both fructose 1,6‐bisphosphate and fructose 2,6‐bisphosphate can largely prevent this activation. Activation can be reversed by dithiothreitol or cysteine. It appears that GSSG activates this enzyme by thiol/disulfide exchanges with the enzyme's specific sulfhydryl groups.