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Tick‐borne encephalitis virus genome
Author(s) -
Pletnev A.G.,
Yamshchikov V.F.,
Blinov V.M.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81160-7
Subject(s) - flavivirus , biology , virology , plasmid , tick borne encephalitis virus , recombinant dna , homology (biology) , dna , gene , virus , microbiology and biotechnology , genome , encephalitis , genetics
RNA of a flavivirus, tick‐borne encephalitis virus (TBEV; strain Sofjin), was subjected to reverse transcription and the DNA copy was transformed into double‐stranded DNA by the action of E . coli DNA‐polymerase I (Klenow fragment). This DNA was annealed with plasmid pBR322. The recombinant plasmids were cloned in E . coli K802. The nucleotide sequence of the inserts of the clones, coding for region structural proteins C, M, E and nonstructural protein NS1, was determined by the Maxam‐Gilbert method. The genes of structural proteins form a compact cluster. Homology has been studied of the TBEV sequences found with the structures of proteins and RNAs of other flaviviruses, yellow fever virus and West Nile virus, and a high degree of homology was found.