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Physico‐chemical study of complex formation of DNA with wild‐type and mutant E. coli RNA polymerases Recognition properties of β‐subunit
Author(s) -
Ozoline O.N.,
Kamzolova S.G.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81155-3
Subject(s) - mutant , protein subunit , wild type , polymerase , chemistry , dna , dna polymerase , rna , microbiology and biotechnology , genetics , biology , biochemistry , gene
Complex formation of T 7 DNA with RNA polymerase from E . coli B/r WU‐36‐10‐11‐12 ( E . coli W 12) and its rifampicin‐resistant mutant rpoB409 was studied. The rpoB409 mutant possesses a highly pleiotropic effect due to alteration in the RNA polymerase β‐subunit structure. The two RNA polymerases have been previously shown to differ in gene selection during RNA synthesis on T 7 DNA. In this study it was found that the change in selective properties of the mutant RNA polymerase occurs during its interaction with DNA, the general ability of the enzyme to melt DNA being unaffected.