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The effect of iron and agar on production of hydrogen peroxide by stimulated and activated mouse peritoneal macrophages
Author(s) -
Thompson H.Lorraine,
Stevenson Joan,
Brock J.H.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81153-x
Subject(s) - hydrogen peroxide , agar , chemistry , microbiology and biotechnology , biochemistry , biology , bacteria , genetics
The effect of iron on H 2 O 2 production by mouse peritoneal macrophages exposed to opsonised zymosan has been investigated. Macrophages elicited with thioglycollate broth produced less H 2 O 2 than macrophages activated by Corynebacterium parvum , and levels were not affected by prior incubation of the cells with 0.1 mM iron nitrilotriacetate. However, preincubation with the iron chelator desferrioxamine (1 mM) reduced H 2 O 2 production by both types of macrophages. Incubation of macrophages with agar, a component of thioglycollate broth, also reduced H 2 O 2 production, particularly by C. parvum ‐activated macrophages. The results indicate that although iron appears to be necessary for H 2 O 2 production by macrophages, the low level of production by thioglycollate‐elicited macrophages is not due to an inadequate level of metabolically utilisable iron, but may be a result of prior ingestion of agar present in the broth.