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Ca 2+ ‐dependent inactivation of acetylcholine receptors by an endogenous transglutaminase
Author(s) -
Hucho F.,
Bandini G.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81152-8
Subject(s) - tissue transglutaminase , endogeny , chemistry , receptor , acetylcholine , biochemistry , biophysics , microbiology and biotechnology , pharmacology , enzyme , biology
The nicotinic acetylcholine receptor (nAChR) from Torpedo californica and T. marmorata electric tissue polymerises irreversibly when DTE and Ca 2+ are added to receptor‐rich membranes. The polymerisation is time‐dependent and complete within 3 h at 30°C. It can be completely prevented by EGTA or the transglutaminase inhibitor cystamine. Transglutaminase activity can also be monitored with the exogenous substrates [ 3 H]putrescine and dimethylcasein. This assay can also be inhibited by EGTA or cystamine.