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Localization and quantitation of opsin and transducin mRNAs in bovine retina by in situ hybridization histochemistry
Author(s) -
Brann Mark R.,
Young W.Scott
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81151-6
Subject(s) - transducin , opsin , in situ hybridization , messenger rna , biology , rhodopsin , microbiology and biotechnology , retina , biochemistry , retinal , gene , neuroscience
Oligodeoxynucleotide probes complementary to a portion of bovine opsin mRNA and transducin mRNA were used for in situ hybridization histochemistry. Within the retina, only photoreceptors expressed mRNAs detectable with these probes, and the majority of both mRNAs were in photoreceptor inner segments. More opsin mRNA was detected than transducin mRNA. In the inner segments 0.54 ± 0.05 copies/μm 3 of opsin mRNA and 0.34 ± 0.05 copies/μm 3 of transducin mRNA were detected. In the outer nuclear layer, 0.39 ± 0.06 copies/ μm 3 of opsin mRNA and 0.27 ± 0.04 copies/μm 3 of transducin mRNA were detected.