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Modulation of cytosolic protein kinase C activity by ferricyanide: priming event seems transmembrane redox signalling
Author(s) -
Malviya A.N.,
Anglard P.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81149-8
Subject(s) - cytosol , chemistry , transmembrane protein , redox , priming (agriculture) , microbiology and biotechnology , ferricyanide , modulation (music) , biochemistry , biophysics , protein kinase a , kinase , biology , enzyme , receptor , inorganic chemistry , physics , botany , germination , acoustics
Transformed 3T3/10T1/2 cultured cells incubated with ferricyanide caused a decrease of 2 mM EDTA extractable cytosolic protein kinase C activity in 2 min, whereas 5 or 20 min ferricyanide treatment reverted the enzyme activity to that observed without ferricyanide. The ferricyanide effect in 2 min was abolished by amiloride and sustained by ouabain. Thus, deactivation‐activation of cytosolic protein kinase C is attributed to an unknown signal generation during H + accumulation coupled with the Na + /H + exchange phase. In this mechanism the priming event concerns the transmembrane redox process shedding H + into the cell interior while impermeant ferricyanide acts as a unique electron acceptor.