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Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides
Author(s) -
Falloon Judith,
Malech Harry,
Milligan Graeme,
Unson Cecilia,
Kahn Richard,
Goldsmith Paul,
Spiegel Allen
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81141-3
Subject(s) - antiserum , pertussis toxin , toxin , g protein , microbiology and biotechnology , biology , bordetella pertussis , antibody , chemistry , biochemistry , bacteria , receptor , immunology , genetics
Antisera raised against the carboxy‐terminal decapeptide (KENLKDCGLF) of transducin‐α detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL‐60 and U937 cells, and revealed an approx. 2‐fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin‐sensitive guanine nucleotide‐binding protein in human leukocytes.