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Amino acid sequence determination of guuanyl‐specific ribonuclease Sa from Streptomyces aureofaciens
Author(s) -
Shiyapnikov S.V.,
Both V.,
Kulikov V.A.,
Dementiev A.A.,
Ševčík J.,
Zeiinka J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81138-3
Subject(s) - edman degradation , streptomyces aureofaciens , s tag , ribonuclease , ribonuclease t1 , ribonuclease iii , peptide sequence , biochemistry , chemistry , protease , amino acid , proteases , streptomycetaceae , microbiology and biotechnology , streptomyces , actinomycetales , biology , enzyme , rnase p , rna , genetics , bacteria , gene , rna interference
Using automated Edman degradation of two nonfractionated peptide mixtures of tryptic and staphylococcal protease digests of the protein, the complete amino acid sequence of the guanyl‐specific ribonuclease Sa from Streptomyces aureofaciens was established. Ribonuclease Sa contains 96 amino acid residues ( M r 10 566). A 50% sequence homology of ribonuclease Sa to the guanyl‐specific ribonuclease St from S . erythrew was found.