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Absence of a unique relationship between active transport of lactose and protonmotive force in E. coli
Author(s) -
Ghazi Alexandre,
Delamourd Lourdes,
Shechter Emanuel
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81136-x
Subject(s) - chemistry , lactose , chemiosmosis , biophysics , biochemistry , biology , atp synthase , enzyme
The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E . coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized chemiosmotic theory, two different relationships were obtained depending on the method used.