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Kurloff cell proteoglycans
Author(s) -
Landemore Gérard,
Letaïef Slim-Eric,
Bocquet Jean,
Izard Jacques
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81131-0
Subject(s) - glycosaminoglycan , chemistry , glucosamine , chromatography , chondroitin sulfate , electrophoresis , biochemistry , proteoglycan , population , in vitro , sepharose , extracellular matrix , enzyme , medicine , environmental health
This paper reports the first direct demonstration of de novo synthesis ofchondroitin sulphate proteoglycans by Kurloff cells. This was achieved using highly purified splenic Kurloff cells labelled in vitro with [ 35 S]sulphate and D‐[U‐ 3 H]glucosamine. A single population of sulphated proteoglycans was observed after dissociative extraction, DEAE‐cellulose chromatography, Sepharose CL 6B chromatography and fluorography after electrophoresis. These were large, highly anionic proteoglycans and were completely digested by chondroitinase AC or ABC. Moreover, glycosaminoglycan extracted from Kurloff cells had the electrophoretic mobility of control chondroitin sulphate.