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Coat protein blocks the in vitro transcription of the virion RNAs of alfalfa mosaic virus
Author(s) -
Houwing Corrie J.,
Jaspars E.M.J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81128-0
Subject(s) - alfalfa mosaic virus , rna , coat protein , transcription (linguistics) , biology , virology , brome mosaic virus , virus , rna polymerase , mosaic virus , microbiology and biotechnology , plant virus , rna dependent rna polymerase , gene , genetics , linguistics , philosophy
Using a method described by Miller and Hall [(1983) Virology 125, 236‐241] for brome mosaic virus, we succeeded in obtaining preparations of an alfalfa mosaic virus specified RNA polymerase that is totally dependent on added RNA. The enzyme makes full‐size transcripts on each of the four virion RNAs. Transcription is inhibited by small amounts of coat protein subunits. This is in accordance with a model proposed by Nassuth and Bol [(1983) Virology 124, 75‐85] which says that in a late stage of the infection cycle the coat protein is a factor that regulates the minus‐strand RNA synthesis in a negative sense.