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Preliminary X‐ray studies of the tetra‐heme cytochrome c 3 and the octa‐heme cytochrome c 3 from Desulfovibrio gigas
Author(s) -
Sieker L.C.,
Jensen L.H.,
LeGall J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81124-3
Subject(s) - heme , cytochrome c , cytochrome , chemistry , desulfovibrio , crystallography , orthorhombic crystal system , polyethylene glycol , hemeprotein , cytochrome c peroxidase , stereochemistry , crystal structure , sulfate , biochemistry , organic chemistry , enzyme , mitochondrion
A tetra‐heme and an octa‐heme cytochrome c 3 from the sulfate bacterium Desulfovibrio gigas have been crystallized. Diffraction quality crystals of the tetra‐heme cytochrome are obtained from solution by the addition of polyethylene glycol at pH 6.5. The crystals are orthorhombic, space group P2 1 2 1 2 with unit cell parameters a = 42.27 Å, b = 52.54 Å and c = 52.83 Å. The octa‐heme cytochrome crystals develop from low ionic strength solutions of phosphate or Tris‐Cl in the pH range 6.2–7.6. The crystals belong to the trigonal system, space group P3 1 or the enantiomorph P3 2 , with unit cell parameters a = b = 57.4 Å, c = 97.3 Å, γ = 120°. Single crystal diffraction studies of the structures of these two low‐potential cytochromes are in progress.