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Cyclooxygenase‐catalyzed formation of 9‐hydroxylinoleic acid by guinea pig alveolar macrophages under non‐stimulated conditions
Author(s) -
Engels F.,
Willems H.,
Nijkamp F.P.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81121-8
Subject(s) - cyclooxygenase , guinea pig , chemistry , incubation , thromboxane , basal (medicine) , linoleic acid , arachidonic acid , biochemistry , thromboxane a2 , thromboxane b2 , medicine , endocrinology , fatty acid , biology , enzyme , platelet , receptor , insulin
Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non‐stimulated conditions 20 × 10 6 AM released about 1 nmol 9‐hydroxylinoleic acid (9‐HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12‐hydroxy‐5,8,10‐heptadecatrienoic acid (HHT), a byproduct of the formation of thromboxane A 2 . 9‐HODE and HHT were the most prominent products formed under the incubation conditions used. These products were formed via a Cyclooxygenase‐catalyzed reaction, since their formation was inhibited by 1 μM indomethacin. Thus, under basal conditions AM possess substantial cyclooxygenase activity. The biological significance of 9‐HODE and related substances is discussed.