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Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP‐dependent protein kinase
Author(s) -
Hemmings Brian A.,
Schwarz Manuela,
Rao Adavani S.,
Jans David A.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81114-0
Subject(s) - complementary dna , cloning (programming) , protein subunit , expression cloning , microbiology and biotechnology , biology , gene , genetics , computer science , programming language
We report here the isolation and sequence of a cDNA for the type II regulatory subunit of the cAMP‐dependent protein kinase (cAMP‐PK) from a λgt‐11 cDNA library derived from a porcine epithelial cell line (LLC‐PK 1 ). The cDNA was detected by immunological screening using an affinity purified polyclonal antibody for bovine R II . DNA sequence analysis of the 467 bp Eco RI insert confirmed the identity of the clone, because the deduced amino acid sequence corresponded to the published sequence for the bovine R Π protein. Northern analysis of total RNA from the LLC‐PK 1 cells indicated a single mRNA species of about 6.0 kb, probably derived from a single copy gene.