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Isolation of a cDNA for the rat heavy neurofilament polypeptide (NF‐H)
Author(s) -
Robinson Philip A.,
Wion Didier,
Anderton Brian H.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81111-5
Subject(s) - complementary dna , isolation (microbiology) , neurofilament , chemistry , microbiology and biotechnology , biochemistry , biology , bioinformatics , immunology , gene , immunohistochemistry
We have isolated from a rat brain λgtll expression library two overlapping cDNA clones of sizes 2.5 and 3.0 kb corresponding to the heavy neurofilament polypeptide (NF‐H). The 2.5 kb insert apparently represents virtually the whole of the C‐terminal tail, the 3.0 kb insert also encodes the conserved epitope for the monoclonal antibody, anti‐IFA. The identity of the cDNAs was established by comparison of the predicted amino acid sequence with the known partial amino acid sequence of porcine NF‐H. A repeat peptide sequence that may be a multiphosphorylation site was identified in the C‐terminal non‐helical tail.