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The second pX product p27 x‐III of HTLV‐1 is required for gag gene expression
Author(s) -
Inoue Jun-ichiro,
Seiki Motoharu,
Yoshida Mitsuaki
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81108-5
Subject(s) - transfection , microbiology and biotechnology , gene , messenger rna , gene product , gene expression , transcription (linguistics) , biology , virology , chemistry , genetics , linguistics , philosophy
The human T‐cell leukemia virus type‐1 (HTLV‐1) contains a unique pX region, which encodes the gene products p40 x , p27 x‐III and p21 x‐III . p40 x is required for transcriptional trans‐activation, whereas p27 x‐III and p21 x‐III have no such function. Transfection of pX expression plasmids containing different combinations for the three gene products into cells integrated with HTLV‐1 proviruses defective in pX expression revealed that both p40 x and p27 x‐III are required for expression of the gag protein and accumulation of gag mRNA. These observations suggest that the pX product p40 x activates transcription and p27 x‐III controls the level of gag mRNA by post‐transcriptional modulation.