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Loss of quin 2 accompanies degranulation of mast cells
Author(s) -
Bibb Philip C.,
Cochrane David E.,
Morel-Laurens Nicole
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81105-x
Subject(s) - degranulation , egta , exocytosis , histamine , chemistry , extracellular , stimulation , biophysics , mast cell , compound 48/80 , calcium , biochemistry , biology , secretion , endocrinology , immunology , receptor , organic chemistry
Stimulation of quin 2 loaded mast cells in the presence of 1 mM extracellular calcium produced a rapid and sustained increase in quin 2 fluorescence. This was accompanied by degranulation and the release of histamine. When Ca was replaced by EGTA or when Mn was present, a decrease in fluorescence accompanied degranulation. The increase in quin 2 fluorescence accompanying stimulation of mast cells appears to be due to the interaction of extracellular Ca with quin 2 associated with the secretory granule matrix released upon exocytosis.