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Inhibition of phorbol ester stimulated superoxide production by 1‐oleoyl‐2‐acetyl‐ sn ‐glycerol (OAG); fact or artefact?
Author(s) -
Bonser R.W.,
Dawson J.,
Thompson N.T.,
Hodson H.F.,
Garland L.G.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81098-5
Subject(s) - superoxide , phorbol , extracellular , chemistry , glycerol , neutrophile , enantiomer , biochemistry , intracellular , microbiology and biotechnology , protein kinase c , stereochemistry , in vitro , enzyme , biology
OAG‐stimulated superoxide (O − 2 ) production by HL‐60 granulocytes showed enantiomeric specificity but reached a maximum of only 5% of that produced by either phorbol myristate acetate (PMA) or phorbol dibutyrate (PDBu). At 10–100 μM, OAG displaced specifically‐bound [ 3 H]PDBu from intact HL‐60 cells by only 25%, suggesting limited cell penetration. OAG (10–100 μM) also inhibited PDBu‐stimulated O 2 production by 25%; this inhibition was enantiomerically specific. However, at a lower concentration (3 μM), both enantiomers of OAG fully blocked O − 2 production stimulated by PMA (0.5 μM). This inhibition is probably artefactual, due to the hydrophobic PMA physically associating with OAG in the extracellular fluid.