Premium
An extremely hydroxyproline‐rich glycoprotein is expressed in inverting Volvox embryos
Author(s) -
Schlipfenbacher Reiner,
Wenzl Stephan,
Lottspeich Friedrich,
Sumper Manfred
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81084-5
Subject(s) - glycoprotein , biochemistry , hydroxyproline , chemistry , arabinose , galactose , amino acid , biology , fermentation , xylose
A sulphated glycoprotein, denoted I‐SG, has previously been shown to be synthesized in Volvox under developmental control [(1982) FEBS Lett. 143, 311‐315]. It is demonstrated that I‐SG synthesis is restricted to a period during embryonic inversion as short as 10 min (out of the 48 h life cycle). This glycoprotein was purified and chemically characterized. 62% of its amino acid residues are hydroxyproline. Clusters of hydroxyproline residues were found to occur in its polypeptide chain. Besides arabinose and galactose, 3‐ O ‐methylgalactose and 6‐ O ‐methylgalactose were identified as constituents of I‐SG.