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Mitotic inhibitors arrest the growth of Plasmodium falciparum
Author(s) -
Usanga E.A.,
O'Brien Estella,
Luzzato L.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81077-8
Subject(s) - plasmodium falciparum , colcemid , schizogony , mitosis , biology , microbiology and biotechnology , chinese hamster , in vitro , mutant , genetics , malaria , immunology , gene
We report that the mitotic inhibitor, vinblastin (VLB), is highly toxic to the malarial parasite, Plasmodium falciparum . In cultures in vitro growth is inhibited by 50% at a VLB level of about 28 nM, and totally abolished at a level of 100 nM. By tests on synchronized cultures we have found that the effect of VLB takes place at the trophozoite stage. Colcemid also inhibits schizogony with somewhat different kinetics. By mutagenesis with nitrosoguanidine followed by VLB selection we have isolated a VLB‐resistant mutant which exhibits cross‐resistance to vincristine. These data suggest a critical role of microtubules in the asexual schizogonic cycle of P. falciparum .