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The formation of inositol phosphate derivatives by isolated membranes from Acer pseudoplatanus is stimulated by guanine nucleotides
Author(s) -
Dillenschneider M.,
Hetherington A.,
Graziana A.,
Alibert G.,
Berta P.,
Haiech J.,
Ranjeva R.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81059-6
Subject(s) - gtp' , guanosine , nucleotide , inositol , chemistry , guanine , acer pseudoplatanus , biochemistry , membrane , nucleoside diphosphate kinase , uridine , inositol phosphate , rna , enzyme , receptor , gene
Membranes isolated from Acer pseudoplatanus cell suspension cultures that were prelabelled with [ 3 H]inositol are able to release different water‐soluble phosphoinositol derivatives in a time‐dependent manner. Addition of adenine, cytidine or uridine nucleotide has no effects on the process. In contrast, guanine nucleotide triphosphates were stimulatory in a dose‐dependent manner. The non‐hydrolyzable derivative guanosine 5'‐[γ‐thio]triphosphate was the most effective activator while guanosine 5'[β‐thio]diphosphate inhibited the action of GTP. It is suggested that GTP‐binding proteins exist in the Acer membrane and play a regulatory role in the formation of potentially important second messengers.