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Mechanism of UDP‐sugar transport into intracellular vesicles
Author(s) -
Cecchelli Roméo,
Cacan René,
Verbert André
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81058-4
Subject(s) - vesicle , intracellular , chemistry , antiporter , nucleotide sugar , nucleotide , cytosol , biochemistry , chromosomal translocation , biophysics , microbiology and biotechnology , membrane , biology , enzyme , gene
The mechanism of translocation of UDP‐GlcNAc, UDP‐Gal and UDP‐Glc into intracellular vesicles has been studied using thymocytes whose plasma membranes have been permeabilized with isotonic ammonium chloride. It has been previously shown that the intracellular vesicles have specific carriers for UDP‐GlcNAc and UDP‐Gal. We now report that the translocation of these two sugar nucleotides occurs via UDP‐GlcNAc/UDP and UDP‐Gal/UDP antiports. The entry of UDP‐GlcNAc or UDP‐Gal into vesicles was specifically dependent on the exit of UDP from these vesicles. In contrast, no antiport mechanism has been recovered with UDP‐Glc for which no transport and accumulation into intracellular vesicles were observed.