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Solubilization and partial purification of UDP‐galactose:diacylglycerol galactosyltransferase activity from spinach chloroplast envelope
Author(s) -
Covès Jacques,
Block Maryse A.,
Joyard Jacques,
Douce Roland
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81057-2
Subject(s) - galactosyltransferase , chemistry , chaps , spinach , diacylglycerol kinase , chromatography , membrane , octyl glucoside , chloroplast , biochemistry , solubilization , galactose , enzyme , fractionation , chloroplast membrane , thylakoid , protein kinase c , gene
We have developed procedures to solubilize the envelope UDP‐galactose:diacylglycerol galactosyltransferase activity and to assay this enzyme after solubilization with a zwitterionic non‐denaturing detergent (CHAPS) and fractionation. From solubilized envelope membranes isolated from intact spinach chloroplasts, we were able to prepare by chromatography on hydroxyapatite a fraction enriched (6–7‐fold) in this enzyme responsible for monogalactosyldiacylglycerol synthesis.