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Inhibition of a calcium‐activated, non‐selective cation channel, in a rat insulinoma cell line, by adenine derivatives
Author(s) -
Sturgess Nicholas C.,
Nicholas Hales C.,
Ashford Michael L.J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81056-0
Subject(s) - chemistry , membrane , adenosine , adenine nucleotide , biophysics , biochemistry , membrane potential , calcium , nucleotide , insulinoma , biology , pancreas , organic chemistry , gene
The effects of adenosine and adenine nucleotides on a calcium‐activated non‐selective cation channel, present in the plasma membrane of an insulin‐secreting cell line CRI‐G1 were investigated. Single‐channel currents were recorded from inside‐out membrane patches and the adenine derivatives applied to the solution bathing the cytoplasmic aspect of the membrane surface. The activity of this channel is shown to be inhibited by all the derivatives tested. The potency sequence for inhibition was found to be AMP > ADP > ATP > adenosine.