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A new crystal form of large ribosomal subunits from Halobacterium marismortui
Author(s) -
Shoham M.,
Müssig J.,
Shevack A.,
Arad T.,
Wittmann H.G.,
Yonath A.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81042-0
Subject(s) - crystallography , crystallization , halobacterium , polyethylene glycol , synchrotron radiation , ribosomal rna , electron microscope , chemistry , crystal (programming language) , materials science , optics , bacteriorhodopsin , physics , biochemistry , organic chemistry , membrane , computer science , gene , programming language
A new form of three‐dimensional crystals of the 50 S ribosomal subunits from Halobacterium marismortui has been obtained at 19°C, using polyethylene glycol in the presence of 1.2–1.7 M KCl in the crystallization mixture. The crystals diffract X‐rays to 13 Å and are stable in the synchrotron radiation beam for 4–8 h. Being aggregates of thin plates, the dimensions of only two unit cell edges, 147 × 181 Å, with an angle of 95°, could so far be determined by both X‐ray crystallography and electron microscopy. Attempts to produce thicker crystals by sophisticated seeding are in progress.