Premium
Oxygen exchange between the Fe(IV)O heme and bulk water for the A 2 isozyme of horseradish peroxidase
Author(s) -
Hashimoto Shinji,
Nakajima Ryo,
Yamazaki Isao,
Tatsuno Yoshitaka,
Kitagawa Teizo
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81038-9
Subject(s) - horseradish peroxidase , heme , isozyme , peroxidase , chemistry , oxygen , hemeprotein , biochemistry , enzyme , organic chemistry
Resonance Raman spectra were observed for compound II of horseradish peroxidase A 2 , and the Fe(IV)O stretching Raman line was identified at 775 cm −1 . This Raman line shifted to 741 cm −1 upon a change of solvent from H 2 16 O to H 2 18 O, indicating occurrence of the oxygen exchange between the Fe(IV)O heme and bulk water. The oxygen exchange took place only at the acidic side of the heme‐linked ionization with p K a = 6.9.