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Human chorionic gonadotropin activates the inositol 1,4,5‐trisphosphate‐Ca 2+ intracellular signalling system in bovine luteal cells
Author(s) -
Davis John S.,
West Leigh A.,
Weakland Laura L.,
Farese Robert V.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81034-1
Subject(s) - inositol , second messenger system , corpus luteum , endocrinology , medicine , inositol phosphate , phospholipase c , luteal phase , human chorionic gonadotropin , intracellular , inositol trisphosphate , gonadotropin , chemistry , cytosol , gonadotropin releasing hormone , biology , luteinizing hormone , hormone , receptor , biochemistry , enzyme
Human chorionic gonadotropin, hCG, a hormone which increases intracellular cAMP, provoked rapid (30 s) and sustained (up to 30 min) increases in the levels of inositol mono‐, bis‐ and trisphosphates (IP, IP 2 and IP 3 , respectively) in bovine luteal cells. LiCl (10 mM) enhanced inositol phosphate accumulation in response to hCG. Concentration‐dependent increases in inositol phosphates, cAMP and progesterone accumulation were observed in hCG‐treated luteal cells. hCG also induced rapid and concentration‐dependent increases in cytosolic free Ca 2+ as measured by quin 2 fluorescence. These findings demonstrate that hCG stimulates the phospholipase C‐IP 3 and diacylglycerol ‘second messenger’ system in the bovine corpus luteum.