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Membrane current responses to intracellular injections of inositol 1,3,4,5‐tetrakisphosphate and inositol 1,3,4‐trisphosphate in NG108‐15 hybrid cells
Author(s) -
Higashida Haruhiro,
Brown David A.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81033-x
Subject(s) - inositol , intracellular , chemistry , inositol phosphate , biophysics , inositol trisphosphate , iontophoresis , inositol trisphosphate receptor , biochemistry , endocrinology , biology , neuroscience , receptor
Iontophoretic injections of inositol 1,4,5‐trisphosphate inside neuroblastoma × glioma NG108‐15 hybrid cells evoked an outward K + current across the outer cell membrane, probably activated by the release of intracellular Ca 2+ . No such current was produced by equivalent intracellular injections of inositol 1,3,4‐trisphosphate or inositol 1,3,4,5‐tetrakisphosphate. Instead, these compounds evoked an inward current with a reversal potential of about −20 mV, and which may therefore be due to a non‐specific cation conductance. This suggests that these derivatives are unable to release sufficient Ca 2+ to activate the Ca 2+ ‐dependent K + current in these cells.