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Atrial natriuretic factor receptors and stimulation of cyclic GMP formation in normal and malignant osteoblasts
Author(s) -
Fletcher A.E.,
Allan E.H.,
Casley D.J.,
Martin T.J.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81029-8
Subject(s) - endocrinology , medicine , osteoblast , calvaria , receptor , stimulation , parathyroid hormone , cyclic gmp , chemistry , biology , calcium , in vitro , biochemistry , nitric oxide
Synthetic rat atrial natriuretic factor (Ile‐ANF‐26) stimulated cyclic GMP formation by up to several hundred‐fold in osteoblast‐rich cultures from newborn rat calvaria and in clonal osteogenic sarcoma cells (UMR 106‐01) which are phenotypically osteoblast. ANF had no effect on the cyclic AMP response to parathyroid hormone in the same cells. Specific, high‐affinity binding sites for ANF were identified in both cell types, with K d and receptor numbers in normal osteoblasts of 1.2 ± 0.1 × 10 −10 M and 42 ± 4 × 10 3 per cell, and in UMR 106‐01 cells of 1.4 ± 0.1 × 10 −10 M and 22 ± 4 × 10 3 per cell.