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A gamma 3 hinge region probe: first specific human immunoglobulin subclass probe
Author(s) -
Huck S.,
Keyeux G.,
Ghanem N.,
Lefranc M.-P.,
Lefranc G.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81022-5
Subject(s) - subcloning , subclass , microbiology and biotechnology , biology , restriction fragment length polymorphism , southern blot , antibody , gene , molecular probe , gamma globulin , genetics , polymerase chain reaction , recombinant dna
We report the first specific human immunoglobulin subclass probe which was obtained by subcloning the gamma 3 hinge region. This specific γ 3 probe allowed us to identify with certainty the Cγ 3 gene on Southern genomic blots to describe the first Cγ 3 restriction fragment length polymorphism (EZZ γ 3 RF) and to show that an IgG3 selective deficiency previously described serologically was not due to a deletion of the C 3 gene. Such a probe should be particularly useful for screening libraries from individuals with IgG3 immunodeficiencies or presenting unusual Cγ 3 genes and consequently for studying the C γ gene evolution. Immunoglobulin IgCH gene ( Human ) Gm allotype Restriction fragment length polymorphism Immunoglobulin G3 deficiency Hinge