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DNA sequence analysis of diuron‐resistant mutations in the mitochondrial cytochrome b gene of Saccharomyces cerevisiae
Author(s) -
di Rago J.-P.,
Perea X.,
Colson A.-M.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81019-5
Subject(s) - cytochrome b , mitochondrial dna , saccharomyces cerevisiae , biology , gene , microbiology and biotechnology , mutant , mitochondrial respiratory chain , cytochrome , yeast , primer (cosmetics) , genetics , mutation , dna , biochemistry , mitochondrion , chemistry , enzyme , organic chemistry
Diuron (3‐[34‐dichlorophenyl]‐1,1‐dimethylurea), an inhibitor of mitochondrial respiration blocks the yeast respiratory chain between cytochrome b and c 1 . Diuron‐resistant mutants of Saccharomyces cerevisiae have been selected and several mutations localized to the mitochondrial cytochrome b gene. The present paper identifies specific DNA base changes within the cytochrome b gene conferring diuron‐resistance. DNA sequence analysis was done utilizing primer extension of crude mitochondrial RNA preparations in the presence of reverse transcriptase. Five independent diuron‐resistant mutations have been sequenced.