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ADP stimulates IP 3 formation in human platelets
Author(s) -
Daniel James L.,
Dangelmaier Carol A.,
Selak Mary,
Smith J.Bryan
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)81000-6
Subject(s) - ionomycin , platelet , phosphorylation , extracellular , chemistry , ionophore , stimulation , biophysics , myosin , adenosine diphosphate , microbiology and biotechnology , biochemistry , medicine , endocrinology , intracellular , biology , platelet aggregation , membrane
Aspirinated human platelets labeled with 32 PO 4 showed a 1.7‐fold increase in [ 32 P]IP 3 when stimulated with ADP. ADP‐stimulated mobilization of internal Ca 2+ and phosphorylation of myosin were enhanced in the presence of extracellular Ca 2+ but the increase in IP 3 was not significantly affected by external Ca 2+ . The Ca 2+ ionophore, ionomycin, elevated internal Ca 2+ and induced myosin phosphorylation without a detectable change in IP 3 . These results indicate that the mechanism of ADP stimulation of human platelets is similar to that of other platelet agonists and supports the theory that IP 3 functions to liberate internal Ca 2+ .