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Synthesis of human des‐tryptophan‐1,norleucine‐12‐minigastrin‐II and its biological activities
Author(s) -
Wünsch E.,
Moroder L.,
Göhring W.,
Borin G.,
Calderan A.,
Bali J.-P.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80981-4
Subject(s) - gastrin , chemistry , norleucine , sulfation , tryptophan , receptor , biological activity , peptide , homogeneous , chromatography , biochemistry , secretion , amino acid , in vitro , physics , methionine , thermodynamics
A human minigastrin‐II analog was prepared by conventional methods in solution using N ‐benzyloxycarbonyltyrosine O ‐sulfate as starting material in the synthetic route. Upon removal of the acid‐labile protecting groups and purification by preparative reversed‐phase chromatography the sulfated gastrin peptide was obtained in satisfactory overall yields as homogeneous material. It was found to be about twice as active as the non‐sulfated form in stimulating gastric acid secretion and to exhibit a 10‐fold higher affinity to gastrin receptors of purified parietal cells.