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In vitro synthesis of catalase protein in sweet potato root microbodies
Author(s) -
Sakajo Shigeru,
Asahi Tadashi
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80924-3
Subject(s) - catalase , edman degradation , biochemistry , in vitro , protein subunit , enzyme , microbody , protein biosynthesis , immunoprecipitation , chemistry , peptide , biology , microbiology and biotechnology , peptide sequence , gene
Poly(A) + RNA prepared from sweet potato root tissue was translated in a wheat germ in vitro translation system. A translation product was immunoprecipitated with anti‐sweet potato catalase immunoglobulin G. The product was identical to the subunit of the catalase with respect to the mobility on an SDS‐polyacryl‐amide gel and the pattern of the peptide map, indicating that the catalase protein is synthesised in vitro in the same size as the mature subunit. No amino acids were released from the purified enzyme protein by Edman degradation, suggesting the occurrence of a minor modification in the N‐terminal part of the protein during the enzyme formation.