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Analysis of globin transition in Xenopus laevis and identification of globins by in vitro translation of hybrid‐selected mRNA
Author(s) -
Sandmeier E.,
Gygi D.,
Wyler T.,
Nyffenegger U.,
Weber R.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80901-2
Subject(s) - globin , metamorphosis , biology , xenopus , messenger rna , complementary dna , microbiology and biotechnology , translation (biology) , larva , polyacrylamide gel electrophoresis , rna , protein biosynthesis , biochemistry , hemoglobin , gene , enzyme , botany
The switch from larval to adult globin synthesis during amphibian metamorphosis was analyzed by polyacrylamide gel electrophoresis in acid urea‐Triton X‐100. 4–6 slowly migrating components were detected in larvae and at least 4 faster components appear at metamorphosis and persist in the adult stage. To relate the globin subunits to the mRNA sequences, isolated as cDNA clones, mRNAs were hybrid‐selected from total RNA of larval and adult erythrocytes and translated in a wheat germ system. The translation products were identified by comparison with the globin pattern of hemolysates