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Regulation of insulin secretion by cAMP in rat islets of Langerhans permeabilised by high‐voltage discharge
Author(s) -
Jones Peter M.,
Fyles Julie M.,
Howell Simon L.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80898-5
Subject(s) - forskolin , medicine , endocrinology , secretion , insulin , islet , egta , phosphodiesterase inhibitor , phosphodiesterase , ibmx , chemistry , adenosine , adenylate kinase , cyclic adenosine monophosphate , insulin oscillation , cyclase , cytosol , biology , calcium , enzyme , biochemistry , receptor , stimulation
Adenosine 3',5‐cyclic monophosphate (cAMP) was shown to stimulate insulin secretion from electrically permeabilised islets of Langerhans incubated in Ca 2+ /EGTA buffers. cAMP‐induced insulin secretion occurred in the presence of either sub‐stimulatory (50 nM) or stimulatory (> 100 nM) concentrations of Ca 2+ . Similar effects on secretion were obtained in response to 8‐bromo‐cAMP (8‐Br‐cAMP) or the phosphodiesterase inhibitor, 3‐isobutyl‐1‐methylxanthine. Forskolin (0.2–20μM) increased adenylate cyclase activity and enhanced insulin secretion from the permeabilised islets. These results suggest that, in electrically permeabilised islets, cAMP‐induced insulin secretion is not dependent on changes in cytosolic Ca 2+ .