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The 66 kDa component of eukaryotic initiation factor 3 interacts with globin mRNA and 18 S rRNA in preinitiation complexes
Author(s) -
Westermann P.,
Sohi M.K.,
Arnstein H.R.V.
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80891-2
Subject(s) - messenger rna , eif4a1 , protein subunit , eukaryotic initiation factor , polyacrylamide gel electrophoresis , initiation factor , transcription preinitiation complex , microbiology and biotechnology , ribosomal rna , biology , eukaryotic small ribosomal subunit , globin , chemistry , biochemistry , enzyme , gene expression , gene , translation (biology) , promoter
The 66 kDa protein present in a complexwith globin mRNA and 18 S rRNA [(1984) Eur. J. Biochem. 143, 27‐33] has been reincorporated into functional eukaryotic initiation factor 3 (eIF‐3) under conditions of protein synthesis. Additionally, two‐dimensional polyacrylamide gel electrophoresis has been used to demonstrate the identity of the 66 kDa protein with the 66 kDa subunit ofeIF‐3.