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Binding of cardiotoxin analogue III from Formosan cobra venom to FL cells
Author(s) -
Takechi Masayuki,
Tanaka Yasuo,
Hayashi Kyozo
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80882-1
Subject(s) - cardiotoxin , venom , amnion , binding site , chemistry , cytotoxicity , binding constant , microbiology and biotechnology , stereochemistry , snake venom , biophysics , biochemistry , biology , fetus , in vitro , pregnancy , genetics
The binding equilibrium at 37 or 0°C of 125 I‐cardiotoxin analogue III (CT III) to fetal lung (FL) cells (cultured human amnion cells) was achieved within 1 h, and the binding at 37°C was irreversible. The Scatchard analysis at 37°C on the binding of 125 I‐CT III indicated that FL cells had two types of binding sites with different association constants. The association constant and the number of high‐affinity sites was 1.1 × 10 10 mol −1 or 2.8 × 10 6 per FL cell, respectively. At 37 or 0°C, the cytotoxicity of CT III paralleled the amount of bound CT III to FL cells, and at 37°C was inhibited by the presence of acidic phospholipids.