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A novel 15 kDa Ca 2+ ‐binding protein present in the eggs of the sea urchin, Hemicentrotus pulcherrimus
Author(s) -
Hosoya Hiroshi,
Iwasa Fuyuki,
Ohnuma Masaaki,
Mabuchi Issei,
Mohri Hideo,
Sakai Hikoichi,
Hiramoto Yukio
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80878-x
Subject(s) - sea urchin , hemicentrotus , calmodulin , binding protein , biochemistry , biology , chemistry , microbiology and biotechnology , enzyme , gene
A novel Ca 2+ ‐binding protein, different from calmodulin, has been purified to homogeneity from the soluble cytoplasmic protein fraction of the egg of the sea urchin, Hemicentrotus pulcherrimus . This protein, designated as 15 kDa protein, shows a Ca 2+ ‐dependent mobility shift upon SDS‐gel electrophoresis and has Ca 2+ ‐binding ability. This protein did not resemble the sea urchin egg calmodulin in either molecular mass or amino acid composition. The 15 kDa protein could not activate cyclic adenosine 3',5'‐monophosphate‐dependent phosphodiesterase from bovine brain and did not bind to fluphenazine‐Sepharose 6B. Antibodies against the 15 kDa protein did not react with sea urchin egg calmodulin. These results suggest that the 15 kDa protein is a novel Ca 2+ ‐binding protein in the sea urchin egg.