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Solution structure of d‐GAATTCGAATTC by 2D NMR
Author(s) -
Hosur R.V.,
Ravikumar M.,
Chary K.V.R.,
Sheth Anu,
Govil Girjesh,
Zu-Kun Tan,
Miles H.Todd
Publication year - 1986
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(86)80868-7
Subject(s) - deoxyribose , vicinal , chemistry , coupling constant , ring (chemistry) , crystallography , resolution (logic) , cleavage (geology) , nuclear magnetic resonance spectroscopy , ribose , stereochemistry , geometry , nucleic acid , physics , materials science , organic chemistry , mathematics , biochemistry , particle physics , artificial intelligence , fracture (geology) , computer science , composite material , enzyme
A new approach based on the correlated spectroscopy (COSY) in 2D NMR has been described for determination of sugar geometries in oligonucleotides. Under the usual low resolution conditions employed in COSY, the intensities of cross peaks depend on the magnitudes of coupling constants. There are five vicinal coupling constants in a deoxyribose ring which are sensitive to the sugar geometry. The presence, absence and rough comparison of relative intensities of COSY cross peaks arising from such coupling constants enable one to fix the sugar conformation to a fair degree of precision. The methodology has been applied to d‐GAATTCGAATTC. It is observed that ten out of the twelve nucleotide units in this sequence exhibit a rare Ol′‐endo geometry. The Eco RI cleavage sites (between G and A) in the dodecanucleotide show an interesting variation in the conformation with the two sugars attached to the Gs acquiring a geometry between C2′‐endo and C4′‐endo.